IGJ 抗体 (Biotin)
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- 抗原 See all IGJ 抗体
- IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IGJ antibody is conjugated to Biotin
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应用范围
- Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)
- 特异性
- Human J chain of dimeric IgA.
- 纯化方法
- Purified IgG
- 免疫原
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Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Fr
Type of Immunogen: Purified protein - Top Product
- Discover our top product IGJ Primary Antibody
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anti-Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (AA 23-159) antibody
IGJ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (AA 1-159) antibodyIGJ 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (AA 32-61), (N-Term) antibodyIGJ 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (AA 45-70) antibodyIGJ 适用: 人, 小鼠, 大鼠 IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: DB, ELISA, ICC, IHC, IHC-P
Usage: In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates. as detection reagent in nonisotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:200 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2. No preservative added.
- 储存液
- Without preservative
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
- 别名
- JCHAIN / Ig J Chain (IGJ 产品)
- 别名
- IGJ antibody, DKFZp469K0627 antibody, IGCJ antibody, JCH antibody, 9530090F24Rik antibody, AI323815 antibody, Jch antibody, joining chain of multimeric IgA and IgM antibody, immunoglobulin joining chain antibody, Jchain antibody, JCHAIN antibody
- 背景
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Name/Gene ID: JCHAIN
Synonyms: JCHAIN, IGCJ, Immunoglobulin J chain, IGJ, IgJ chain, JCH - 基因ID
- 3512
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