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- 抗原
- Robo3.2
- 抗原表位
- AA 1303-1316
- 适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- 非结合性
- 应用范围
- ELISA, Immunohistochemistry (IHC)
- 原理
- Robo3.2 (mouse aa1303-1316)
- 序列
- KESQGRGRGL EACR
- 交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- KESQGRGRGLEACR
- 亚型
- IgG
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-
- 应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.
Western Blot: Preliminary experiments gave bands at approx 85 kDa in Mouse fetal Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of
Peptide ELISA: antibody detection limit dilution 1:128000.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- Robo3.2
- 背景
- Robo3, roundabout homolog 3 (Drosophila), Rbig1, Rig-1, Rig1, Robo3a, Robo3b, retinoblastoma inhibiting gene 1, retinoblastoma-inhibiting gene 1 protein, roundabout homolog 3
- 基因ID
- 19649
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