The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
免疫原
TOB1 (phospho Ser164) antibody was raised against a peptide sequence around phosphorylation site of Serine 164 (A-V-S (p)-P-T) derived from Human TOB1.
This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.