PsbO 抗体
适用: Spinach, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii
WB
宿主: 兔
Polyclonal
unconjugated
1 image
(1 reference)-
- 抗原
- PsbO
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适用
- Spinach, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB)
- 预测反应
- dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 35 / 33 kDa
- 纯化方法
- affinity purified
- 免疫原
- native purified 33 kDa protein from Spinacia oleracea UniProt P12359
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anti-PsbO antibody
适用: Arabidopsis thaliana, Barley, Nicotiana tabacum WB, IP, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).
- 说明
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This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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: "Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT." in: Bioresource technology, Vol. 143, pp. 154-62, (2013) (PubMed).
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: "Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT." in: Bioresource technology, Vol. 143, pp. 154-62, (2013) (PubMed).
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- 抗原
- PsbO
- 背景
- PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
- 分子量
- expected: 35 kDa, apparent: 33 kDa
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