PCNA antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 10 μg/mL.
限制
仅限研究用
状态
Liquid
缓冲液
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
As with all antibodies avoid freeze/thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Store PCNA antibody at 4 °C or -20 °C.
抗原
PCNA
(Proliferating Cell Nuclear Antigen (PCNA))
别名
PCNA
背景
The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species.