Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
KPNA6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
KPNA6 antibody was raised against a 14 amino acid synthetic peptide near the internal of KPNA6.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 60 kDa band observed in human skeletal muscle lysates (calculated MW of 60.0 kDa according to NP_036448.1). Recommended concentration:0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human adrenal gland shows staining of cytoplasm and some nuclear membrane (nuclear pores). Recommended concentration, 3-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.