Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
S100A9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
S100A9 antibody was raised against a 15 amino acid synthetic peptide near the internal region of S100A9.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 16 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 13.2 kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15 kDa ) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280 (50) :41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows nuclear staining in select cells, Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.