This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
纯化方法
GAD67 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
GAD67 antibody was raised against a 12 amino acid synthetic peptide near the internal region of GAD67.
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 70 kDa band observed in human cerebellum and in mouse and rat brain lysates (calculated MW of 66.9 kDa according to human NP_000808.2 and 66.6 kDa according to mouse NP_032103.2). Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.