This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
纯化方法
PNPLA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PNPLA3 antibody was raised against a 12 amino acid synthetic peptide near the internal region of PNPLA3.
PNPLA3
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 555
应用备注
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 45 kDa band observed in human heart lysates (calculated MW of 42.6 kDa according to NP_473429.2). Recommended concentration: 0.03-0.1 μg/mL. Approximately 45 kDa band observed in mouse heart lysates (calculated MW of 42.6 kDa according to human NP_473429.2 and to mouse NP_473429.2). Recommended concentration: 0.05-0.2 μg/mL. Immunohistochemistry: In paraffin embedded human Skin shows membranous staining of basak kayer cells. Recommended concentration, 4-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.