Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
特异性
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
纯化方法
VPS35 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
VPS35 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of VPS35.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 90 kDa band observed in human brain and testis lysates (predicted MW of 91 kDa according to NP_060676.2). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.