Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize isoform 1 (NP_057292.1) only.
纯化方法
VPS28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
VPS28 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of VPS28.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 24 kDa band observed in H460, human testis and brain lysates (predicted MW of 25 kDa according to NP_057292.1). Recommended for use at 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.