Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
VPS25 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
VPS25 antibody was raised against a 10 amino acid synthetic peptide near the internal region of VPS25.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))