Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
TXNDC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TXNDC1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of TXNDC1.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 35 kDa band observed in human liver lysates (calculated MW of 31.8 kDa according to NP_110382). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human spleen shows textured staining in the cytoplasm of some splenocytes. Recommended concentration, 3-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
TMX1
(Thioredoxin-Related Transmembrane Protein 1 (TMX1))