Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
纯化方法
TRPV5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRPV5 antibody was raised against a 12 amino acid synthetic peptide near the internal region of TRPV5.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 85 kDa band observed in human pancreas lysates (calculated MW of 82.6 kDa according to NP_062815.2). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.