Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
TOM1L2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TOM1L2 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of TOM1L2.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 60 kDa band observed in human Fibroblast A431 lysates (calculated MW of 51.8 kDa according to NP_653279). Recommended concentration: 0.1-1 μg/mL. IHC: In paraffin embedded Human Skeletal Muscle shows variable staining from fibre to fibre. Recommended concentration, 5-10μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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