SV2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SV2A antibody was raised against a 13 amino acid synthetic peptide near the internal region of SV2A.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band observed in rat brain lysates (calculated MW of 82.6 kDa according to NP_476558.1 (rat). Recommended concentration: 0.3-1 μg/mL. an additonal band of unknown identity was also consistently observed at 28 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.