Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
This antibody is expected to recognize all 5 isoforms of SCARF1. Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.
纯化方法
SREC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SREC antibody was raised against an 11 amino acid synthetic peptide near the internal region of SREC.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 85-90 kDa band observed in human heart lysates (calculated MW of 87.4 kDa according to NP_003684,). Recommended concentration: 0.3-1.0 μg/mL. an additional faint band of 28 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.