Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
SH3BP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SH3BP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SH3BP1.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human lung and kidney lysates (predicted MW of 71 kDa according to NP_061830). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.