PDK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PDK1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of PDK1.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 65-70 kDa band observed in rat heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))