This antibody is expected to recognize all six Human isoforms of this protein (NP_683702.1 and NP_683703.1 are varients which represent the same isoform).
纯化方法
ORP9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ORP9 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of ORP9.
OSBPL9
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 647
应用备注
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 150 kDa and 19 kDa in lysates of human brain, and testis and 55 kDa in lysates of human placenta and Testes after 0.5 μg/mL antibody staining.5 kDa for NP_683702.1 and NP_683703.1, b: 63.9 kDa for NP_683704.1, c: 70.2 kDa for NP_683705.1, d: 81.8 kDa for NP_683706.1, e: 83.2 kDa for NP_078862.2, f: 84.3 kDa for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human lung shows staining of alveolar macrophages.. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.