This antibody is expected to recognize all three human isoforms of this protein.
纯化方法
Nup50 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Nup50 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of Nup50.
NUP50
适用: 大鼠
ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in Jurkat lysate (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.