NFIL3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
NFIL3 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of NFIL3.
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 55-60 kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5 kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18,94 (6) :2609-14., PMID: 9122243). Recommended concentration: 0.5-1.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.