MLL4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
MLL4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of MLL4.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave no signal but low background in human heart lysates at up to 1 μg/mL. However this used our routine Western Blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approximately 312 kDa according to NP_055542. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human testis shows cytoplasm staining of spermatids. Recommended concentration, 5-10 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.