Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
MDA5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
MDA5 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of MDA5.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: No signal obtained yet but low background observed in human placenta lysate at up to 1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
IFIH1
(Interferon Induced with Helicase C Domain 1 (IFIH1))