Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
纯化方法
GPX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
GPX3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of GPX3.
GPX3
适用: 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 488
应用备注
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band observed in mouse kidney and liver lysates (calculated MW of 27.7 kDa according to mouse NP_001077398.1 [isoform1], 25.4 kDa according to mouse NP_032187.2 [isoform 2] and 25.5 kDa according to human NP_002075.2). Recommended concentration: 1-3 μg/mL. IHC: Frozen sections of Porcin Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration, 20μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.