Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.1) and isoform III (NP_683697.1 and NP_683698.1).
纯化方法
FOXP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
FOXP2 antibody was raised against a 14 amino acid synthetic peptide near the internal region of FOXP2.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 85 kDa band observed in human brain (cerebellum and frontal cortex) lysates and in rat brain lysates (calculated MW of 82.6 kDa according to NP_683696.1). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 28-30 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human prostate shows nuclear staining in the secretory cells. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.