Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
ENPP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ENPP1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of ENPP1.
ENPP1
适用: 人
ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution 1:32000. Western Blot: In transfected HEK293 transiently expressing ENPP1 a band of approximately 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105 kDa according to NP_006199.2. Recommended concentration, 1-3 μg/mL. Immunohistochemistry: In paraffin embedded liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on human pancreas showing membranous staining of acinar cells. Recommended concentration, 1-2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.