Western Blotting (WB), ELISA, Immunoprecipitation (IP)
特异性
This antibody is expected to recognize both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
纯化方法
ELMO1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ELMO1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ELMO1.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 70 kDa and 50 kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2 μg/mL. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa ). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.