This antibody is expected to recognize EHD1 protein as well as EHD2.
纯化方法
EHD2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
EHD2 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of EHD2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in human heart lysates (calculated MW of 61.2 kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approximately 62 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.003-0.01 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.