Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is not expected to cross-react with other DGAT members.
纯化方法
DGAT2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DGAT2 antibody was raised against a 14 amino acid synthetic peptide near the internal region (near the C-Terminus) of DGAT2.
DGAT2
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 488
应用备注
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: In transfected HEK293 transiently expressing DGAT2 bands of approximately 60 kDa and 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in human liver lysates. The calculated molecular size is 43.8 kDa according to NP_115953.2. Recommended concentration, 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.