Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
纯化方法
DDX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DDX5 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of DDX5.
DDX5
适用: 人, 小鼠, 大鼠
WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 70 kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1 kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.