This antibody is expected to recognize both reported isoforms (NP_005704.1 and NP_112729.1)
纯化方法
COL4A3BP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
COL4A3BP antibody was raised against an 11 amino acid synthetic peptide near the internal region of COL4A3BP.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in mouse heart lysates (calculated MW of 71.1 kDa according to mouse NP_075909.1 and 60.0 kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 26 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
COL4A3BP
(Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP))