Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody is expected to recognize all four reported isoforms (NP_001002273, NP_001002274, NP_001002275, NP_003992)
纯化方法
CD32 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CD32 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of CD32.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 34 kDa band observed in human myeloid erythroblast cell line K562 and human monocytic cell line U937 lysates (calculated MW of 34.0 kDa according to NP_003992.3,). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in human placenta. Recommended concentration, 3-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.