All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1)
纯化方法
BNIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
BNIP1 antibody was raised against a 15 amino acid synthetic peptide near the internal region of BNIP1.
ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 28 kDa band observed in human kidney lysates (calculated MW of 26.1 kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
BNIP1
(BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))