Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
BAF57 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
BAF57 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of BAF57.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 50 kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
SMARCE1
(SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))