Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
纯化方法
ARP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ARP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ARP1.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 45 kDa band observed in human heart and brain lysates (predicted MW of 43 kDa according to NP_005726). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.