APPL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
APPL1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of APPL1.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 80 kDa band observed in rat Aortic Smooth muscle Cells (lane 1), intensity increased after infection with human aPPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.