The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
纯化方法
ALMS1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ALMS1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ALMS1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 200 kDa and 75 kDa in lysates of human T cell line Jurkat after 0.3 μg/mL antibody staining.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.