Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
Both variants (NP_006057.1and NP_697021.1) represent identical protein
纯化方法
Aldehyde Reductase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Aldehyde Reductase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Aldehyde Reductase.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 38 kDa band observed in human placenta and liver lysates (calculated MW of 36.6 kDa according to NP_006057.1). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows Brush Border staining in PCT. Recommended concentration, 2-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
AKR1B1
(Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))