ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize isoforms 1, 2 and 3 (NP_671700.1, NP_005742.4, NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
纯化方法
AKAP9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
AKAP9 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of AKAP9.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 450 kDa band observed in HEPG2 lysate (calculated MW of 454 kDa according to NP_ 671700.1). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
AKAP9
(A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))