BIF-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
BIF-1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of BIF-1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 38 kDa band observed in lysates of human T cell line Jurkat and in human lung and placenta (calculated MW of 40.8 kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 μg/mL. A minor band of unknown identity was also consistently observed at 50 kDa in Jurkat and placenta. This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
ZBTB24
(Zinc Finger and BTB Domain Containing 24 (ZBTB24))