Pyruvate Dehydrogenase (C-Term) 抗体
适用: 人
WB, IHC, ELISA
宿主: 山羊
Polyclonal
unconjugated
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- 抗原
- Pyruvate Dehydrogenase
- 抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- This antibody is expected to recognise all reported isoforms.
- 纯化方法
- Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 免疫原
- Pyruvate Dehydrogenase antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Pyruvate Dehydrogenase.
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- 应用备注
- ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 500 μg/mL
- 缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20 °C.
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