Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
KIF5B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
KIF5B antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of KIF5B.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 120 kDa band observed in human kidney lysates (calculated MW of 110 kDa according to NP_004512.1). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration, 1-2 μg/mL. In paraffin embedded human uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration, 2-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.