Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Enzyme Immunoassay (EIA)
纯化方法
GFAP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
GFAP antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of GFAP.
GFAP
适用: 人
WB, IHC, IF
宿主: 兔
Monoclonal
unconjugated
应用备注
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50 kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 49.9 kDa according to Mouse NP_034407.2 and Human NP_002046.1). Recommended concentration: 0.3-1 g/mL. IHC: In paraffin embedded Human Cerebelum shows specific staining of astrocytes. Recommended concentration, 2-4 g/mL. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GFAP captured by a rabbit antibody. Recommended reporter concentration: 5-10 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.