This antibody is expected to recognize both human isoforms of this protein.
纯化方法
DAP3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DAP3 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of DAP3.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 40 kDa band observed in HeLa lysate (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approximately 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.