Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
Catalase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Catalase antibody was raised against a 14 amino acid synthetic peptide near the internal region of Catalase.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in human kidney and liver lysates (calculated MW of 59.8 kDa according to NP_001743.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.