This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.
纯化方法
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DAPP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of DAPP1.
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band in human heart lysates after 1 μg/mL antibody staining.0 kDa according to AAF14578.1. The 26 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 30 kDa band observed in human kidney lysates (calculated MW of 32.0 kDa according to AAF14578.1). Recommended concentration: 0.5-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
DAPP1
(Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))