RBM20 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
RBM20 antibody was raised against an 11 amino acid synthetic peptide near the internal region of RBM20 (aa 717-727).
Approximately 150 kDa band observed in mouse heart lysates (calculated MW of 130 kDa according to mouse NP_001164318.1). Recommended concentration: 1-3 μg/mL. an additional band of 25 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.