CIRBP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
CIRBP antibody was raised against an 11 amino acid synthetic peptide near the internal region of CIRBP (aa 81-91).
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 18 kDa band observed in mouse and rat testis lysates (calculated MW of 18.6 kDa according to mouse NP_031731.1 and rat NP_112409.2). Recommended concentration: 0.05-0.2 μg/mL. an additional band of unknown identity was also consistently observed at 23 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
CIRBP
(Cold Inducible RNA Binding Protein (CIRBP))