This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).
纯化方法
POU5F1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
POU5F1B antibody was raised against a 14 amino acid synthetic peptide near the internal region (near C-Terminus) of POU5F1B (near C-Terminus).
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 28 kDa band in human heart lysates after 1 μg/mL antibody staining.6 kDa according to NP_001153014.1. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.